Characterization of single-file flow through human retinal parafoveal capillaries using an adaptive optics scanning laser ophthalmoscope
نویسندگان
چکیده
Adaptive Optics Scanning Laser Ophthalmoscopy was used to noninvasively acquire videos of single-file flow through live human retinal parafoveal capillaries. Videos were analyzed offline to investigate capillary flow dynamics. Certain capillaries accounted for a clear majority of leukocyte traffic (Leukocyte-Preferred-Paths, LPPs), while other capillaries primarily featured plasma gap flow (Plasma-Gap-Capillaries, PGCs). LPPs may serve as a protective mechanism to prevent inactivated leukocytes from entering exchange capillaries, and PGCs may serve as relief valves to minimize flow disruption due to the presence of a leukocyte in a neighboring LPP.
منابع مشابه
Adaptive optics scanning laser ophthalmoscopy.
We present the first scanning laser ophthalmoscope that uses adaptive optics to measure and correct the high order aberrations of the human eye. Adaptive optics increases both lateral and axial resolution, permitting axial sectioning of retinal tissue in vivo. The instrument is used to visualize photoreceptors, nerve fibers and flow of white blood cells in retinal capillaries.
متن کاملWhat can adaptive optics do for a scanning laser ophthalmoscope ?
By compensating for the aberrations in the eye that cause blur, the adaptive optics scanning laser ophthalmoscope (AOSLO) yields high-magnification, high-resolution, real-time images of the living human retina. Features as small as single cone photoreceptors can be resolved, single leukocytes are recorded in real time as they pass through the smallest retinal capillaries, and the optical sectio...
متن کاملNoninvasive visualization and analysis of parafoveal capillaries in humans.
PURPOSE To demonstrate a noninvasive method to visualize and analyze the parafoveal capillary network in humans. METHODS An adaptive optics scanning laser ophthalmoscope was used to acquire high resolution retinal videos on human subjects. Video processing tools that enhance motion contrast were developed and applied to the videos to generate montages of parafoveal retinal capillaries. The ca...
متن کاملIntroduction: Feature Issue on Cellular Imaging of the Retina
The editors introduce the Biomedical Optics Express feature issue, “Cellular Imaging of the Retina,” which includes 14 contributions from the vision and optics community. ©2011 Optical Society of America OCIS codes: (000.1200) Announcements, awards, news, and organizational activities; (110.1080) Active or adaptive optics; (110.2960) Image analysis; (170.0170) Medical optics and biotechnology; ...
متن کاملSubclinical capillary changes in non-proliferative diabetic retinopathy.
PURPOSE To establish adaptive optics scanning laser ophthalmoscopy as a method to detect and characterize microscopic signs of diabetic retinopathy in capillaries and cone photoreceptors in the parafovea. METHODS Recently, adaptive optics scanning laser ophthalmoscope (AOSLO) has enabled noninvasive assessment of photoreceptors, capillaries, and leukocytes in the retinas of live human subject...
متن کامل